{"id":236,"date":"2018-07-19T23:27:00","date_gmt":"2018-07-19T20:27:00","guid":{"rendered":"http:\/\/2019.brs.org.tr\/?page_id=236"},"modified":"2018-07-29T17:09:43","modified_gmt":"2018-07-29T14:09:43","slug":"konfokal-mikroskop","status":"publish","type":"page","link":"https:\/\/2020.brs.org.tr\/index.php\/konfokal-mikroskop\/","title":{"rendered":"Konfokal Mikroskop"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-237\" src=\"http:\/\/2019.brs.org.tr\/wp-content\/uploads\/2018\/07\/kon-2.jpg\" alt=\"\" width=\"1600\" height=\"900\" srcset=\"https:\/\/2020.brs.org.tr\/wp-content\/uploads\/2018\/07\/kon-2.jpg 1600w, https:\/\/2020.brs.org.tr\/wp-content\/uploads\/2018\/07\/kon-2-300x169.jpg 300w, https:\/\/2020.brs.org.tr\/wp-content\/uploads\/2018\/07\/kon-2-768x432.jpg 768w, https:\/\/2020.brs.org.tr\/wp-content\/uploads\/2018\/07\/kon-2-1024x576.jpg 1024w\" sizes=\"auto, (max-width: 1600px) 100vw, 1600px\" \/><\/p>\n<p><strong>Konfokal lazer taramal\u0131 mikroskop;<\/strong><\/p>\n<p>Floresan mikroskoplarda y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc g\u00f6r\u00fcnt\u00fc sayesinde h\u00fccrelerin yap\u0131lar\u0131n\u0131 daha net bir \u015fekilde g\u00f6rebilir. Konfokal lazer taramal\u0131 mikroskop ta ise bu g\u00f6r\u00fcnt\u00fcler daha net bir \u015fekilde al\u0131nabilmektedir. Di\u011fer bir ifade ile ince doku \u00f6rnekleri ve h\u00fccrelerden 3-D g\u00f6r\u00fcnt\u00fcleri elde etmek m\u00fcmk\u00fcnd\u00fcr. \u00d6rne\u011fin, h\u00fccre i\u00e7i serbest Ca+2 miktar\u0131n\u0131 belirlemek i\u00e7in, de\u011fi\u015fik floresan boylar ile (Fura-2, Flo-3 ve Fluo-8) ink\u00fcbe edilen h\u00fccreler, konfokal lazer taramal\u0131 mikroskop alt\u0131na konulup g\u00f6r\u00fcnt\u00fcletilmektedir. Di\u011fer bir ifade ile h\u00fccre i\u00e7eresindeki g\u00f6r\u00fcnmeyen de\u011fi\u015fik \u00fcr\u00fcnler, anyonlar, katyonlar ve h\u00fccre yap\u0131lar\u0131 g\u00f6r\u00fcn\u00fcr bir hale getirilebilmekte ve miktarlar\u0131 \u00f6l\u00e7\u00fclebilmektedir. Konfokal lazer taramal\u0131 mikroskop temel k\u0131s\u0131mlar\u0131 \u015funlard\u0131r; lazer, \u0131\u015f\u0131n ay\u0131r\u0131c\u0131, taray\u0131c\u0131, objektifler ve lensler, Z kontrol sitemi, \u0131\u015f\u0131k yolu ve foto \u00e7o\u011falt\u0131c\u0131 t\u00fcp. ARGE merkezimizde son model konfokal lazer taramal\u0131 mikroskop (Zeiss, LSM800) mevcut olup uygulamalar bu mikroskop \u00fczerinde g\u00f6sterilecektir.<\/p>\n<h3><strong>Konfokal Mikroskop Kurs Program\u0131<\/strong><\/h3>\n<p>1. Ders Konfokal mikroskop nedir?<br \/>\n2. Ders Konfokal mikroskop i\u00e7in h\u00fccrelerin haz\u0131rlanmamas\u0131, g\u00f6zlenmesi ve floresan boyalar<br \/>\n3. Ders H\u00fccre i\u00e7i Ca+2 analizi ve hesaplanmas\u0131 (Fura-2, flo-3 ve fluo-8)<br \/>\n4. Ders H\u00fccre i\u00e7i apoptosis (Anneksin V ve PI) g\u00f6r\u00fcnt\u00fclenmesi ve hesaplanmas\u0131<br \/>\n5. Ders H\u00fccre i\u00e7i mitokondriyal depolarizasyon (JC-1) g\u00f6r\u00fcnt\u00fclenmesi ve hesaplanmas\u0131<br \/>\n6. Ders H\u00fccre i\u00e7i ROS (Rhodamine 123) g\u00f6r\u00fcnt\u00fclenmesi ve tayini<br \/>\n7. Ders H\u00fccre i\u00e7i glutatyon g\u00f6r\u00fcnt\u00fclenmesi ve tayini<br \/>\n8. Ders Sonu\u00e7lar\u0131n yorumlanmas\u0131<br \/>\n9. Ders Sonu\u00e7lar makale de nas\u0131l kullan\u0131l\u0131r?<br \/>\n10. Ders Tart\u0131\u015fma ve Tavsiyeler<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Konfokal lazer taramal\u0131 mikroskop; Floresan mikroskoplarda y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc g\u00f6r\u00fcnt\u00fc sayesinde h\u00fccrelerin yap\u0131lar\u0131n\u0131 daha net bir \u015fekilde g\u00f6rebilir. Konfokal lazer taramal\u0131 mikroskop ta ise bu g\u00f6r\u00fcnt\u00fcler daha net bir \u015fekilde al\u0131nabilmektedir. Di\u011fer bir ifade ile ince doku \u00f6rnekleri ve h\u00fccrelerden 3-D g\u00f6r\u00fcnt\u00fcleri elde etmek m\u00fcmk\u00fcnd\u00fcr. \u00d6rne\u011fin, h\u00fccre i\u00e7i serbest Ca+2 miktar\u0131n\u0131 belirlemek i\u00e7in, de\u011fi\u015fik floresan boylar ile (Fura-2, Flo-3 ve Fluo-8) ink\u00fcbe edilen h\u00fccreler, konfokal lazer taramal\u0131 mikroskop alt\u0131na konulup g\u00f6r\u00fcnt\u00fcletilmektedir.&hellip; <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-236","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/comments?post=236"}],"version-history":[{"count":3,"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/236\/revisions"}],"predecessor-version":[{"id":439,"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/236\/revisions\/439"}],"wp:attachment":[{"href":"https:\/\/2020.brs.org.tr\/index.php\/wp-json\/wp\/v2\/media?parent=236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}